MCL Research on Unsupervised Nuclei Segmentation
Nuclei segmentation is a consequential task in biological image analysis, helping in the reading process of histology images. Different attributes, such as shape, population, cluster formation and density play a significant role in clinical practice for cancer diagnosis and its aggressiveness level assessment. Given that the annotation of this data is carried out by expertized pathologists who reportedly [2] need to spend on average 120-150 hours to annotate 50 image patches (about 12M pixels), one can realize that annotated data are in scarcity. That is a big impediment for supervised methods, particularly for DL-based solutions that need massive annotated data to learn generalizable representations. Moreover, the annotations have a high inter-observer variation which is subject to the experience of the annotator [1]. On top of that, nuclei color and texture variations across images from different laboratories and multiple organs further widen the gap between train and test domains.
Given the aforementioned limitations, a natural way to solve the problem is to pursue an unsupervised line of research. Also, given the limited number of annotated data, our proposed method decouples from the DL paradigm and utilizes conceptually simpler techniques that make the pipeline more transparent in terms of segmentation decision making. It is mainly based on prior knowledge about the nuclei segmentation problem. CBM [3] pipeline starts out with a data-driven Color (C) transform, to highlight the nuclei cell regions over the background, followed by an adaptive Binarization (B) process built on the bi-modal assumption in each local region. That process is being run in a patch-wise manner, to leverage the local distribution assumptions between background and foreground. The final part of the pipeline uses Morphological (M) transformations that refines the segmented output based on certain [...]